Differentiation of Tilletia Species by rep-PCR Genomic Fingerprinting
نویسندگان
چکیده
منابع مشابه
DNA Fingerprinting Based on Repetitive Sequences of Iranian Indigenous Lactobacilli Species by (GTG)5- REP-PCR
Background and Objective: The use of lactobacilli as probiotics requires the application of accurate and reliable methods for the detection and identification of bacteria at the strain level. Repetitive sequence-based polymerase chain reaction (rep-PCR), a DNA fingerprinting technique, has been successfully used as a powerful molecular typing method to determine taxonomic and phylogenetic relat...
متن کاملApplicability of rep-PCR fingerprinting for identification of Lactobacillus species.
PCR amplification of repetitive bacterial DNA elements fingerprinting using the (GTG)(5) primer ((GTG)(5)-PCR) was proven to be useful for differentiation of a wide range of lactobacilli (i.e. 26 different (sub)species) at the species, subspecies and potentially up to the strain level. Using this rapid and reproducible genotypic technique, new Lactobacillus isolates recovered from different typ...
متن کاملGenomic fingerprinting of Bartonella species by repetitive element PCR for distinguishing species and isolates.
Repetitive-element PCR (rep-PCR) with primers based on repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC) repeated DNA sequences was used for genomic finger-printing of Bartonella species. This technique was applied by using either extracted genomic DNA or preparations of whole bacterial cells directly. PCR fingerprints with either the REP-based p...
متن کاملGenetic relationships between sympatric populations of Bacillus cereus and Bacillus thuringiensis, as revealed by rep-PCR genomic fingerprinting.
The bacterial strain Bacillus cereus is closely related to Bacillus thuringiensis, although any genetic relationship between the two strains is still in debate. Using rep-PCR genomic fingerprinting, we established the genetic relationships between Brazilian sympatric populations of B. cereus and B. thuringiensis simultaneously collected from two geographically separate sites. We observed the fo...
متن کاملgenetic evaluation of indigenous rhizobia nodulating of three clover species, based on rep- pcr fingerprinting method
evaluation of genetic diversity is a considerable approach to screen within indigenous rhizobial population for compatible, highly effective strains, which can be further utilized as rhizobial inoculums. genetic diversity of rhizobium isolates from three clover species grown in soil samples of seven different geographical regions in iran was investigated by repetitive extra genomic palindromic ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Disease
سال: 2000
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis.2000.84.10.1121